David Shoubridge picked up where he left off as he stormed to the top of the timesheets ahead of Tom Tunstall, with a hot lap of 1m:10.502s.
Max Lofthouse was the third quickest as the series returned after a month off, ahead of Andre Compton with Mark Bridger fifth.
Ducati Performance TriOptions Cup, Donington Park, Free Practice One:
1. David Shoubridge, Rich Energy Yamaha
2. Tom Tunstall, Motorcycle Race Parts +0.794s
3. Max Lofthouse, JDF Racing +01.345s
4. Andre Compton, (no team listed) +1.761s
5. Mark Bridger, Moto46 +1.840s